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Uncovering the role of aquaporin and chromobox family members as potential biomarkers in head and neck squamous cell carcinoma via integrative multiomics and in silico approach

  • 0Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, 110025, India.
Journal of Applied Genetics +

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February 15, 2024

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February 15, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Aquaporins (AQPs) and Chromobox (CBX) proteins show altered expression in head and neck squamous cell carcinoma (HNSC). CBX3 and AQP3 may serve as prognostic biomarkers and potential therapeutic targets for HNSC.

Area Of Science

  • Oncology
  • Molecular Biology
  • Biochemistry

Background

  • Head and neck squamous cell carcinoma (HNSC) presents significant morbidity.
  • Aquaporins (AQPs) and Chromobox (CBX) proteins are implicated in various cancers.
  • Understanding their role in HNSC is crucial for developing new therapeutic strategies.

Purpose Of The Study

  • To analyze the expression, prognostic value, and mutational landscape of AQPs and CBXs in HNSC.
  • To investigate the functional pathways and immune microenvironment associations of key AQPs and CBXs.
  • To construct a regulatory network and explore protein-protein interactions involving AQP3 and CBX3.

Main Methods

  • Bioinformatic analysis of AQP and CBX expression, prognosis, and mutations in HNSC.
  • Gene enrichment analysis (GO pathways) and tumor immune infiltration analysis.
  • Construction of a miRNA-TF-mRNA regulatory network and protein-protein docking.

Main Results

  • Upregulation of CBX3/2 and downregulation of AQP3/5/7 correlated with poor overall survival in HNSC.
  • AQP3 and CBX3 were associated with specific cellular functions and modulated immune cell infiltration and tumor purity.
  • A regulatory network identified SMAD3, miR-423-5p, and AQP3 as a key motif, with direct AQP3-Smad3 protein interaction suggested.

Conclusions

  • AQP3 and CBX3 are potential prognostic biomarkers and therapeutic targets for HNSC.
  • The identified regulatory network and protein interactions provide insights into HNSC pathogenesis.
  • Further research into AQP3-Smad3 interactions could elucidate novel therapeutic avenues.

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