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Updated: Jul 3, 2025
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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Published on: March 16, 2017
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Protocol for a placenta-on-a-chip model using trophoblasts differentiated from human induced pluripotent stem cells
Agathe Lermant1, Gwenaëlle Rabussier2, Lindsey Davidson3
1Systems Medicine, School of Medicine, University of Dundee, DD1 9SY Dundee, UK.
STAR Protocols
|February 15, 2024
Summary
This study presents a 3D placenta-on-a-chip model using human induced pluripotent stem cells (hiPSCs). This advanced organoid model enables functional barrier assays and molecular analysis for improved human placenta research.
Area of Science:
- Biomedical Engineering
- Stem Cell Biology
- Organoid Technology
Background:
- Organ-on-a-chip systems offer advanced human organ modeling.
- Stem cell technology enhances the development of these models.
- Placental modeling is crucial for understanding pregnancy and disease.
Purpose of the Study:
- To develop a standardized 3D placenta-on-a-chip model.
- To utilize human induced pluripotent stem cells (hiPSCs) for trophoblast generation.
- To establish a platform for functional and molecular analysis of the placenta.
Main Methods:
- Seeding hiPSCs into multi-chip OrganoPlate devices.
- On-chip differentiation of hiPSCs into trophoblasts.
- Perfused culture conditions with extracellular matrix.
- Functional barrier integrity assays, immunostaining, and molecular analysis (protein/RNA).
Main Results:
- Successful generation of a 3D placenta-on-a-chip model.
- Demonstrated differentiation of hiPSCs into functional trophoblasts.
- Established protocols for barrier integrity and molecular analysis on-chip.
Conclusions:
- The developed placenta-on-a-chip model provides a robust platform for studying placental biology.
- This model facilitates research into placental development and disease.
- The protocol enables standardized and reproducible analysis of placental function.
