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Updated: Jul 3, 2025

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
Published on: November 21, 2013
DNA three-way junction structures with amino acid side chains prepared via post-synthetic amide condensation
Yusuke Takezawa1, Naofumi Kudo1, Nobuyuki Fuse1
1Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Abstract:
DNA three-way junction (3WJ) structures with three amino acid side chains in the core have been synthesized via post-synthetic DNA modification. Amide condensation reactions of oligonucleotides containing 2'-aminouridine with activated esters yielded DNA strands modified with His, Cys and Asp side chains to form modified 3WJs. Even a 3WJ with three negatively charged Asp side chains formed stably at room temperature. Furthermore, DNA hybridization alone placed two (His and Asp) and three (His, Cys, and Asp) side chains within the 3WJs, indicating that the DNA 3WJs are a useful platform for spatial arrangement of amino acid side chains.
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