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Related Experiment Videos

Interferon induction in mouse fibroblast L-A9 cells.

M C Soares, N Volkmer, I C Frugulhetti

    Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
    |January 1, 1985
    PubMed
    Summary

    Mouse L-A9 cells effectively produce interferon (IFN) when stimulated by Newcastle disease virus. This interferon inhibits RNA virus replication, showing potential for antiviral research.

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    Area of Science:

    • Immunology
    • Virology
    • Cell Biology

    Background:

    • Interferons (IFNs) are crucial antiviral proteins.
    • Mouse L-A9 cells are a potential system for IFN production and study.

    Purpose of the Study:

    • To characterize interferon production in mouse L-A9 cells.
    • To evaluate the antiviral efficacy of L-A9 cell-derived interferon against RNA viruses.

    Main Methods:

    • Interferon induction using Newcastle disease virus in L-A9 cells.
    • Partial purification of interferon via ammonium sulphate precipitation and chromatography.
    • Assessment of antiviral activity through viral plaque reduction assays.

    Main Results:

    • High interferon yield (1.5 X 10^5 IU/10^7 cells) achieved.

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  • Purified interferon exhibited specific activity of 1.7 X 10^7 IU/mg protein.
  • Interferon treatment inhibited reovirus replication and showed dose-dependent plaque reduction for multiple viruses.

    • Conclusions:

    • Mouse L-A9 cells represent a viable system for interferon production.
    • L-A9 cell-derived interferon demonstrates broad-spectrum antiviral activity against RNA viruses.
    • These cells serve as a valuable model for studying cellular responses to interferon and viral replication.