Identification of mouse mammary adipose cells by membrane antigens

Antisera produced to mammary adipose cells from midpregnant BALC/c females can be used to distinguish mammary adipose cells from mammary epithelial cells and fibroblasts. The mammary adipose membrane antigen detected by indirect immunofluorescence was found in adipose cells from (a) mammary glands of virgin, midpregnant and lactating mice; (b) mammary fat pads that had been surgically cleared of glandular elements; and (c) epididymis. In all tissues, this cell-surface antigen was removed by the enzymatic action used to dissociate the cells from the tissues and was shown to be fully restored when cells were cultured for 48 hr.