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Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in
Sara J Thygesen1, Sabrina S Burgener2, Prerna Mudai1
1School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Australia.
European Journal of Immunology
|February 16, 2024
Abstract:
Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.

