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Related Concept Videos

Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

248
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
248
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
564

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Routine application of SFC-MS in doping control: Analysis of 3 × 1000 urine samples using three different SFC-MS

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Area of Science:

  • Analytical Chemistry
  • Forensic Science
  • Biochemistry

Background:

  • Supercritical fluid chromatography-mass spectrometry (SFC-MS) is increasingly recognized for its analytical capabilities.
  • The anti-doping community is exploring novel techniques for drug detection in athletes.
  • Current anti-doping strategies rely on gas and liquid chromatography coupled with mass spectrometry.

Purpose of the Study:

  • To evaluate the applicability of SFC-MS for routine anti-doping control.
  • To assess the performance of SFC-MS instruments from multiple vendors for doping analysis.
  • To determine the suitability of SFC-MS as a complementary technique in anti-doping laboratories.

Main Methods:

  • Analysis of approximately 3000 identical anti-doping samples using SFC-MS.
  • Utilized SFC-MS instruments from Agilent Technologies, Waters Corporation, and Shimadzu Corporation.
  • Employed a 'dilute and inject' approach, with and without hydrolysis of glucuronide metabolites.

Main Results:

  • Most compounds exhibited excellent chromatographic separation.
  • Some analytes required mass spectrometry (MS) discrimination due to co-elution with endogenous interferences.
  • Stable retention times (CV ≤ 0.5%) and good, symmetrical peak shapes were observed.
  • All three tested SFC-MS instrument setups proved fit for purpose in anti-doping testing.

Conclusions:

  • SFC-MS is a sensitive, reproducible, and robust analytical technique.
  • SFC-MS is ready for integration into routine anti-doping laboratory workflows.
  • SFC-MS can effectively complement existing chromatographic and mass spectrometric methods for doping control.