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Related Concept Videos

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A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix
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High pressure pasteurization: Simultaneous native tissue decellularization and sterilization.

Akihisa Otaka1, Takashi Yamamoto2, Tetsuji Yamaoka1

  • 1Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-Shimmachi, Suita, Osaka, 564-8565, Japan.

Regenerative Therapy
|February 16, 2024
PubMed
Summary

High hydrostatic pressurization (HHP) effectively sterilizes decellularized xenografts using repeated cycles and antibiotic mediums. This optimized HHP process is crucial for clinical applications of tissue-derived products.

Keywords:
BiomaterialsDecellularizationHigh hydrostatic pressureSterilizationXenografts

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Area of Science:

  • Biomaterials Science
  • Microbiology
  • Sterilization Technologies

Background:

  • Terminal sterilization is essential for the clinical use of decellularized xenografts.
  • High hydrostatic pressurization (HHP) is a potential method for xenograft decellularization and decontamination, but its sterilization efficacy is not well understood.

Purpose of the Study:

  • To investigate the disinfection effectiveness of HHP at 36°C against resilient spore-forming bacteria.
  • To evaluate the impact of repeated versus sustained pressurization and antibiotic-containing media on bacterial elimination.

Main Methods:

  • Spore-forming bacteria (Bacillus atrophaeus, Geobacillus stearothermophilus) were subjected to HHP under repeated and sustained pressurization protocols.
  • Experiments were conducted in a pressurization medium, with and without antibiotic agents.

Main Results:

  • Sustained pressurization, typically used for decellularization, showed limited bacterial elimination.
  • Repeated pressurization significantly enhanced disinfection efficacy.
  • The addition of antibiotics to the pressurization medium further improved disinfection, achieving sterilization levels.

Conclusions:

  • Optimized HHP, utilizing repeated pressurization and antibiotic-containing media, can effectively sterilize biological tissues during decellularization.
  • This method presents a promising strategy for the production of sterile tissue-derived healthcare products.