Related Experiment Video
Updated: Jul 11, 2026
Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
Published on: June 2, 2017
Exploring the efficacy of tryptone-stabilized silver nanoparticles against respiratory tract infection-causing
Pooja Pandey1, Sristi Pradhan1, Kimaya Meher1
1School of Biological Sciences, UM-DAE Centre for Excellence in Basic Sciences, University of Mumbai, Vidyanagari, Kalina Campus, Mumbai 400098, India.
Abstract:
Respiratory tract infections (RTIs) are a common cause of mortality and morbidity in the human population. The overuse of antibiotics to overcome such infections has led to antibiotic resistance. The emergence of multidrug resistant bacteria is necessitating the development of novel therapeutic techniques in order to avoid a major global clinical threat. Our study aims to investigate the potential of tryptone stabilised silver nanoparticles (Ts-AgNPs) on planktonic and biofilms produced byKlebsiella pneumoniae(K. pneumoniae)and Pseudomonas aeruginosa(P. aeruginosa). The MIC50of Ts-AgNPs was found to be as low as 1.7 μg ml-1and 2.7 μg ml-1forK. pneumoniae and P.aeruginosarespectively. Ts-AgNPs ability to alter redox environment by producing intracellular ROS, time-kill curves showing substantial decrease in the bacterial growth and significantly reduced colony forming units further validate its antimicrobial effect. The biofilm inhibition and eradication ability of Ts-AgNPs was found to be as high as 93% and 97% in both the tested organisms. A significant decrease in the eDNA and EPS quantity in Ts-AgNPs treated cells proved its ability to successfully distort the matrix and matured biofilms. Interestingly Ts-AgNPs also attenuated QS-induced virulence factors production. This study paves way to develop Ts-AgNPs as novel antibiotics against RTIs causing bacterial biofilms.
Insights
Tryptone stabilized silver nanoparticles (Ts-AgNPs) show potent antimicrobial activity against Klebsiella pneumoniae and Pseudomonas aeruginosa. These nanoparticles effectively inhibit and eradicate bacterial biofilms, offering a novel therapeutic approach for respiratory tract infections.
Area of Science:
- Nanotechnology
- Microbiology
- Infectious Diseases
Background:
- Antibiotic resistance is a growing global health crisis, driven by the overuse of antibiotics for respiratory tract infections (RTIs).
- Multidrug-resistant bacteria pose a significant threat, necessitating the development of novel therapeutic strategies.
- Bacterial biofilms contribute to persistent infections and antibiotic resistance.
Purpose of the Study:
- To investigate the antimicrobial potential of tryptone stabilized silver nanoparticles (Ts-AgNPs) against Klebsiella pneumoniae and Pseudomonas aeruginosa.
- To evaluate the efficacy of Ts-AgNPs in inhibiting and eradicating bacterial biofilms.
- To explore the mechanism of action of Ts-AgNPs, including their effect on virulence factors.
Main Methods:
- Determined the minimum inhibitory concentration (MIC50) of Ts-AgNPs against planktonic bacteria.
- Assessed the antimicrobial effect using time-kill curves and colony-forming unit counts.
- Evaluated biofilm inhibition and eradication capabilities, and analyzed extracellular DNA (eDNA) and extracellular polymeric substance (EPS) quantity.
- Investigated the impact of Ts-AgNPs on quorum sensing (QS)-induced virulence factors.
Main Results:
- Ts-AgNPs exhibited low MIC50 values (1.7 μg ml⁻¹ for K. pneumoniae, 2.7 μg ml⁻¹ for P. aeruginosa).
- Demonstrated significant reduction in bacterial growth and colony-forming units, indicating potent antimicrobial activity.
- Achieved high biofilm inhibition (up to 93%) and eradication (up to 97%) rates.
- Showed a decrease in eDNA and EPS, disrupting biofilm structure, and attenuated QS-induced virulence factors.
Conclusions:
- Ts-AgNPs possess significant antimicrobial and anti-biofilm properties against K. pneumoniae and P. aeruginosa.
- The nanoparticles disrupt biofilm matrix and reduce bacterial virulence, suggesting a novel mechanism of action.
- Ts-AgNPs hold promise as a new class of antibiotics for treating RTIs caused by bacterial biofilms.
More Related Videos
11:19Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
Published on: May 10, 2018
11:52Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
