Thermal stability and secondary aggregation of self-limiting, geometrically frustrated assemblies: Chain assembly of...

In geometrically frustrated assemblies, equilibrium self-limitation manifests in the form of a minimum in the free energy per subunit at a finite, multisubunit size which results from the competition between the elastic costs of frustration within an assembly and the surface energy at its boundaries. Physical realizations-from ill-fitting particle assemblies to self-twisting protein superstructures-are capable of multiple mechanisms of escaping the cumulative costs of frustration, resulting in unlimited equilibrium assembly, including elastic modes of "shape flattening" and the formation of weak, defective bonds that screen intra-assembly stresses. Here we study a model of one-dimensional chain assembly of incommensurate "polybricks" and determine its equilibrium assembly as a function of temperature, concentration, degree of shape frustration, elasticity, and interparticle binding, notably focusing on how weakly cohesive, defective bonds give rise to strongly temperature-dependent assembly. Complex assembly behavior derives from the competition between multiple distinct local minima in the free-energy landscape, including self-limiting chains, weakly bound aggregates of self-limiting chains, and strongly bound, elastically defrustrated assemblies. We show that this scenario, in general, gives rise to anomalous multiple aggregation behavior, in which disperse subunits (stable at low concentration and high temperature) first exhibit a primary aggregation transition to self-limiting chains (at intermediate concentration and temperature) which are ultimately unstable to condensation into unlimited assembly of finite-chains through weak binding beyond a secondary aggregation transition (at low temperature and high concentration). We show that window of stable self-limitation is determined both by the elastic costs of frustration in the assembly as well as energetic and entropic features of intersubunit binding.