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Updated: Jul 2, 2025

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
Published on: February 4, 2021
Prediction of temperature-dependent nucleation and growth rates from crystallization-related heat release
Joseph Kangas1, Christopher J Hogan1
1Department of Mechanical Engineering, University of Minnesota. 111 Church St SE, Minneapolis MN 55455, USA.
Abstract:
We propose a method for determining the time and, therefore, temperature-dependent relative nucleation and growth rates during crystallization. We do so by linking the partial differential equation governing the time dynamics of the crystal size distribution to kinetic (Avrami) parameters describing heat release. This approach is tested in silico by nucleating and growing diffusion limited aggregates with time-varying morphology and growth rates unhindered by impingement. The associated heat release is analyzed, showing that nucleation and growth rates could be extracted with high fidelity.
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