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Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber
Amy G Harris1, Vico Schot1, Michele Carrabba1
1Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK.
STAR Protocols
|February 17, 2024
Summary
This study presents a novel protocol for decellularizing porcine tissue using a 3D printed flow chamber. This method enhances tissue graft viability for pediatric congenital heart disease surgery.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Cardiovascular Surgery
Background:
- Tissue grafts are vital for treating pediatric congenital heart disease.
- Decellularization reduces graft immunogenicity, offering an alternative to glutaraldehyde fixation.
- Porcine tissues are a potential source for allografts.
Purpose of the Study:
- To present a detailed protocol for decellularizing porcine right ventricular outflow tracts.
- To utilize a 3D printed flow chamber for enhanced decellularization efficiency.
- To provide a method for characterizing the resulting acellular scaffold.
Main Methods:
- Fabrication of a 3D printed flow rig.
- Preparation of porcine right ventricular outflow tract tissue.
- Application of shear forces via the flow rig for decellularization.
- Characterization of the decellularized extracellular matrix scaffold.
Main Results:
- Successful decellularization of porcine right ventricular outflow tracts.
- Demonstration of a reproducible protocol using a 3D printed flow system.
- Characterization data confirming scaffold integrity.
Conclusions:
- The developed protocol offers a viable method for producing decellularized tissue grafts.
- 3D printed flow chambers can enhance decellularization efficiency and reproducibility.
- This technique holds promise for improving surgical outcomes in pediatric congenital heart disease.
