Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber
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Updated: Jul 2, 2025
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
Published on: December 6, 2012
Amy G Harris1, Vico Schot1, Michele Carrabba1
1Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK.
Abstract:
Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber. We describe steps for 3D printing the flow rig, preparing porcine tissue, and using the flow rig to utilize shear forces for decellularization. We then detail procedures for characterizing the acellular scaffold. For complete details on the use and execution of this protocol, please refer to Vafaee et al.1.
