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Probing human immune cell mechanics using acoustic force spectroscopy.

Tom M J Evers1, Mehrad Babaei1, Alireza Mashaghi1

  • 1Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands; Laboratory for Interdisciplinary Medical Innovations, Centre for Interdisciplinary Genome Research, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands.

STAR Protocols
|February 17, 2024
PubMed
Summary

This study details a protocol for measuring the mechanical properties of human monocytes using acoustic force spectroscopy (AFS). This method aids in understanding immune cell mechanics in health and disease.

Keywords:
BiophysicsBiotechnology and bioengineeringImmunology

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Area of Science:

  • Biophysics
  • Immunology
  • Cellular Mechanics

Background:

  • Immune cells require adaptable mechanical properties for circulation and immune responses.
  • Characterizing cell mechanics is crucial for understanding physiological and pathological conditions.

Purpose of the Study:

  • To provide a detailed protocol for assessing single-cell mechanical properties of primary human monocytes.
  • To enable the characterization of immune cell mechanics using acoustic force spectroscopy (AFS).

Main Methods:

  • Step-by-step protocol for acoustic force spectroscopy (AFS) on primary human monocytes.
  • Includes AFS chip calibration, monocyte isolation from buffy coats, and mechanical probing.
  • Details the data analysis strategy for AFS measurements.

Main Results:

  • A comprehensive protocol for probing monocyte mechanics is established.
  • The method allows for detailed analysis of single-cell mechanical properties.

Conclusions:

  • The presented AFS protocol is valuable for characterizing human monocyte mechanics.
  • This methodology can be applied to various immune cells across different physiological and pathological states.