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Osiris17 is essential for stable integrin localization and function during insect wing epithelia remodeling.

Wei Dong1, Chen-Yang Song1, Meng-Qi Liu1

  • 1Research Institute of Applied Biology, Shanxi Key Laboratory of Nucleic Acid Biopesticides, Shanxi University, Taiyuan, Shanxi, China.

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Osiris17 protein is crucial for maintaining cell adhesion in developing insect wings by regulating beta-PS integrin localization and function, impacting epithelial remodeling.

Keywords:
Basal junctionEpithelia remodelingIntegrinOsirisWing

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • Dynamic cell-extracellular matrix adhesion is vital for organ development.
  • Epithelial sheets in insect wings utilize beta-PS integrins for basal adhesion.

Purpose of the Study:

  • Investigate the role of Osiris17 in insect wing development.
  • Determine Osiris17's contribution to beta-PS integrin localization and function.

Main Methods:

  • Studied Osiris17 function in Drosophila and locust wing development.
  • Utilized immunofluorescence microscopy to assess protein localization.
  • Examined endosomal markers Rab5 and Rab11 in relation to Osiris17 and integrins.

Main Results:

  • Reduced Osiris17 expression disrupts epithelial integrity and basal adhesion.
  • Osiris17 co-localizes with endosomal markers Rab5 and Rab11.
  • Osiris17 depletion affects endosome numbers and integrin localization.

Conclusions:

  • Osiris17 is an endosome-associated protein essential for insect wing morphogenesis.
  • Osiris17 facilitates basal integrin localization, maintaining epithelial adhesion and development.