Reliability, stability during long-term storage, and intra-individual variation of circulating levels of osteopontin, osteoprotegerin, vascular endothelial growth factor-A, and interleukin-17A
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Summary
This summary is machine-generated.Reliable measurement of osteopontin (OPN) and vascular endothelial growth factor-A (VEGF-A) is possible in population studies. Interleukin-17A (IL-17A) is only suitable for small experiments, and osteoprotegerin (OPG) requires cautious assessment due to degradation and variability.
Area Of Science
- Biomarker Discovery and Validation
- Population Health Studies
- Analytical Chemistry and Laboratory Medicine
Background
- Circulating cytokine levels are associated with various health conditions.
- Reliability of cytokine measurements in large population studies, including sample stability and individual variation, is not well-established.
Purpose Of The Study
- To assess the analytical reliability of repeated cytokine measurements over short and long terms.
- To evaluate the stability of cytokines during long-term sample storage.
- To determine intra-individual variability of cytokines over seasonal changes.
Main Methods
- Assessed analytical reliability and stability of four cytokines: osteopontin (OPN), osteoprotegerin (OPG), vascular endothelial growth factor-A (VEGF-A), and interleukin-17A (IL-17A).
- Utilized commercial kits for plasma/serum sample measurements following standard procedures.
- Employed random or mixed effects linear models on the log scale for data analysis.
Main Results
- OPN, OPG, and VEGF-A showed acceptable short-term reliability (CV < 0.11); IL-17A demonstrated poor reliability (CVs 0.85/0.43).
- OPG decayed significantly during long-term storage (-33% per year), while OPN and VEGF-A were stable.
- OPN and VEGF-A exhibited low within-individual variation (ICC 0.68/0.83), whereas OPG showed high variation (ICC 0.11).
Conclusions
- OPN and VEGF-A are suitable for reliable measurement in large population studies.
- IL-17A is appropriate only for small-scale experiments due to poor reliability.
- OPG requires cautious interpretation due to storage degradation and significant intra-individual variability.
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