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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Jul 2, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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[Advances in CRISPR sensing and detection technology].

Jiahui Wang1, Peipei Zhao1, Mengfei Qin2

  • 1Biology Insititute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, Shandong, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|February 19, 2024
PubMed
Summary
This summary is machine-generated.

CRISPR sensing and detection technology offers a cheap, simple, and highly sensitive method for molecular diagnostics. This review explores various CRISPR-Cas systems and their applications for detecting diverse targets, promoting future advancements.

Keywords:
biosensorsclustered regulatory interspaced short palindromic repeats (CRISPR)instant detectionmolecular diagnostics

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Diagnostics

Background:

  • CRISPR sensing and detection technology is recognized as a next-generation molecular diagnostic tool due to its cost-effectiveness, simplicity, portability, high sensitivity, and specificity.
  • CRISPR-Cas systems leverage specific recognition, cis-cleavage, and nonspecific trans-cleavage activities for detecting a wide range of targets.

Approach:

  • This review categorizes and analyzes current CRISPR sensing and detection technologies based on the activity characteristics of different Cas proteins.
  • It examines the advantages and developmental history of various CRISPR sensing and detection methodologies.

Key Points:

  • CRISPR systems can detect both nucleic acid targets (DNA, RNA) and non-nucleic acid targets, including proteins, exosomes, cells, and small molecules.
  • Understanding the distinct Cas protein activities is crucial for optimizing CRISPR-based detection platforms.

Conclusions:

  • The review aims to foster a deeper understanding of CRISPR sensing and detection technologies to promote their further development and application.
  • It provides a comprehensive overview of applications across different target types, facilitating the creation of novel CRISPR sensing detection technologies.