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Published on: March 24, 2014
IL-27 Levels in Bronchoalveolar Lavage Fluid in Children with Post-infectious Bronchiolitis Obliterans
Wenjing Liu1, Yiyao Zhang1, Xia Chen2
1Maternal and Child Health Hospital of Hubei Province Training Base, Hubei University of Medicine, Wuhan, Hubei Province, China.
Background:
Pulmonary neutrophils may play a crucial role in the development of bronchiolitis obliterans (BO) following measles virus infection. IL-27 could potentially have a negative regulatory effect on the release of reactive oxygen species and cytotoxic granules in neutrophils.
Objective:
To investigate the levels of IL-27 in the bronchoalveolar lavage fluid (BALF) of children with post-infectious bronchiolitis obliterans (PIBO) and analyze the relationship between IL-27 levels and neutrophil proportions.
Methods:
A total of 24 children with PIBO were recruited for the experimental group, while 23 children with bronchial foreign bodies were included in the control group. Bronchoscopic alveolar lavage was performed in both groups. The levels of IL-27 in BALF were measured using enzyme-linked immunosorbent assay (ELISA). The proportions of neutrophils in BALF were determined by smear staining. The relationship between the levels of IL-27 in BALF and the neutrophil proportions was analyzed by the Pearson test.
Results:
The levels of IL-27 in BALF were significantly lower in children with PIBO compared to children with bronchial foreign bodies (p<0.05). Additionally, the proportions of neutrophils in BALF were significantly higher in children with PIBO compared to children with bronchial foreign bodies (p<0.05). The levels of IL-27 were negatively correlated with the neutrophil proportions in BALF in children with PIBO (p<0.05), but not in children with bronchial foreign bodies (p>0.05).
Conclusion:
The present study suggests that a decrease in IL-27 may be associated with an increase in neutrophils in BALF and may contribute to the pathogenesis of PIBO.
Insights
Lower levels of Interleukin-27 (IL-27) in children with post-infectious bronchiolitis obliterans (PIBO) correlate with increased pulmonary neutrophils, suggesting a role in PIBO pathogenesis.
Area of Science:
- Pediatric Pulmonology
- Immunology
- Infectious Diseases
Background:
- Pulmonary neutrophils are implicated in post-measles bronchiolitis obliterans (BO).
- Interleukin-27 (IL-27) may inhibit neutrophil-mediated inflammation.
Purpose of the Study:
- To quantify IL-27 levels in bronchoalveolar lavage fluid (BALF) of children with post-infectious bronchiolitis obliterans (PIBO).
- To examine the association between IL-27 levels and neutrophil proportions in PIBO patients.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) measured IL-27 in BALF from 24 PIBO patients and 23 controls.
- Neutrophil proportions were assessed via smear staining.
- Pearson test analyzed the correlation between IL-27 and neutrophils.
Main Results:
- PIBO patients exhibited significantly lower BALF IL-27 levels compared to controls (p<0.05).
- Neutrophil proportions were significantly higher in PIBO patients (p<0.05).
- A negative correlation was found between IL-27 levels and neutrophil proportions in PIBO patients (p<0.05).
Conclusions:
- Reduced IL-27 levels may contribute to increased neutrophils in PIBO.
- This suggests a potential role for IL-27 in the pathogenesis of PIBO.
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