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X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

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Fixed Target Serial Data Collection at Diamond Light Source

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Guide to serial synchrotron crystallography.

¹College of General Education, Kookmin University, Seoul, 02707, Republic of Korea.

Current Research in Structural Biology

|February 19, 2024

Summary

Serial synchrotron crystallography (SSX) enables noncryogenic macromolecular structure determination and observation of molecular dynamics. This technique advances structural biology by adapting serial crystallography methods for broader research accessibility.

Keywords:

Radiation damage Room temperature Serial crystallography Serial synchrotron crystallography Time-resolved study X-ray crystallography

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Last Updated: Jul 2, 2025

Fixed Target Serial Data Collection at Diamond Light Source

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Published on: February 26, 2021

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

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Published on: September 23, 2020

$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

Area of Science:

Structural Biology
Biophysics

Background:

Serial crystallography (SX) determines macromolecular structures without freezing, minimizing radiation damage.
SX techniques like pump-probe and mix-and-inject allow observation of time-resolved molecular dynamics.
Serial femtosecond crystallography (SFX) demonstrated SX feasibility using X-ray free electron lasers.

Purpose of the Study:

To review the background, advantages, and experimental approaches of serial synchrotron crystallography (SSX).
To highlight SSX's potential for advancing structural biology research.
To discuss key considerations for implementing SSX experiments.

Main Methods:

Adaptation of serial crystallography (SX) techniques for synchrotron light sources.
Utilizing pump-probe or mix-and-inject methods for time-resolved studies.
Focus on noncryogenic crystal structure determination and radiation damage mitigation.

Main Results:

Serial synchrotron crystallography (SSX) has been successfully developed and implemented.
SSX provides new opportunities for studying macromolecular structure and function.
The technique facilitates deeper understanding of molecular reactions and dynamics.

Conclusions:

SSX is a valuable advancement in structural biology, building upon SX principles.
The method offers enhanced accessibility to SX techniques for a wider research community.
Further research and careful experimental design are crucial for maximizing SSX's impact.