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Updated: Jul 2, 2025
A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Quantitative real-time PCR analysis of four genetically modified soybean events using plasmid and genomic DNA
Min Ki Shin1,2, Seon Min Jeon2, Jong Seok Park2
1Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 08826 Republic of Korea.
Abstract:
A calibration curve is required for reliable and accurate quantitative real-time PCR analysis of genetically modified (GM) organisms, necessitating the use of reference materials as calibrators. In this study, two types of DNA calibrators-plasmid DNA (pDNA) and genomic DNA (gDNA)-were used to quantify four GM soybean events (SYHT0H2, MON87751, DAS-44406-6, and DAS-81419-2). The PCR efficiency and linearity for the calibrators adhered to the CODEX guidelines. The conversion factor (Cf) was calculated as the ratio of copies of GM events to those of endogenous genes using the pDNA calibration curve. To assess the accuracy and repeatability of these assays, quantification at GM levels of 3% and 1% was performed. Based on our results, we believe that the pDNA calibrator assessed in this study can be used as a reference material for GMO quantitative analysis and can replace gDNA, especially considering the ease of management and advantages of mass production.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10068-023-01392-0.
