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Updated: Jul 2, 2025
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
Published on: November 22, 2017
Protocol for preparation and staining of chromosomes isolated from mouse and human tissues for conventional and
Regina Lichti Binz1, Kennedi Burns1, Rupak Pathak1
1Division of Radiation Health, Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Abstract:
The study of chromosomes without or with molecular DNA probes provides crucial insight for understanding research findings, as well as refining diagnosis, prognosis, and therapeutics in clinical settings. Here, we present a protocol for chromosome preparation, conventional G-banding, locus-specific fluorescent in situ hybridization, and spectral karyotyping for both mouse and human samples. This protocol optimizes the preparation of chromosomes from mouse and human cells for subsequent conventional and molecular cytogenetic analysis. For complete details on the use and execution of this protocol, please refer to Binz et al.1.
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