Search research articles

Related Concept Videos

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Home
Detection Of Effusion Tumor Cells Under Different Storage And Processing Conditions.

Home
Detection Of Effusion Tumor Cells Under Different Storage And Processing Conditions.

Related Experiment Video

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Published on: November 5, 2020

Detection of effusion tumor cells under different storage and processing conditions.

Diane M Libert¹, Yili Zhu¹, Aihui Wang^1,2

¹Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.

Cancer Cytopathology

|February 19, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Effusion tumor cells (ETCs) can be detected using various slide processing and storage methods, expanding their clinical utility. Morphology may become more critical due to altered immunofluorescence signals.

Keywords:

RareCyte effusion tumor cells processing rare cell detection storage

More Related Videos

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Published on: December 27, 2017

Related Experiment Videos

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Published on: November 5, 2020

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Published on: December 27, 2017

Area of Science:

Oncology
Cell Biology
Medical Diagnostics

Background:

Circulating tumor cells (CTCs) in blood offer prognostic value.
An established assay detects effusion tumor cells (ETCs) using immunofluorescence on ThinPrep (TP) slides.
Evaluating alternative conditions is crucial for integrating ETC detection into clinical practice.

Purpose of the Study:

To assess the feasibility of detecting effusion tumor cells (ETCs) under varied slide processing and storage conditions.
To determine if alternative methods maintain the reliability of the established ETC detection assay.
To identify optimal conditions for broader clinical application of ETC detection.

Main Methods:

Enumeration of ETCs using the RareCyte platform with defined epithelial cellular adhesion molecule (EpCAM) and CD45 mean fluorescence intensity (MFI) cutoffs.

Analysis of malignant pleural fluid from patients under seven processing/staining conditions and varying storage durations (short-term and long-term at -80°C).

Comparison of MFI values for 4',6-diamidino-2-phenylindol, cytokeratin, CD45, and EpCAM across different conditions.

Main Results:

ETCs were successfully detected across all tested processing and storage conditions.
Ethanol-fixed, unstained TP slides showed the highest similarity to the established air-dried protocol.
While ETCs were detectable after long-term storage, significant differences in marker MFIs were observed, suggesting a greater reliance on morphology.

Conclusions:

Detection of ETCs is feasible under diverse processing and storage scenarios, enhancing potential clinical integration.
Morphological assessment may gain importance in ETC identification due to potential alterations in immunofluorescence signatures.
The study supports the adaptability of the ETC detection assay for wider use in clinical settings.