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RNF115 aggravates tumor progression through regulation of CDK10 degradation in thyroid carcinoma
Jinxiang Zhu1,2, Longwei Guo3, Hao Dai1
1Department of Otorhinolaryngology-Head and Neck Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Western Yanta Road, Xi'an City, 710061, Shaanxi Province, China.
Cell Biology and Toxicology
|February 20, 2024
Summary
RING Finger Protein 115 (RNF115) promotes thyroid carcinoma (THCA) progression and metastasis by degrading CDK10. This E3 ligase activates the Raf-1 pathway, driving cancer cell proliferation and cell cycle advancement in THCA.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- RING Finger Protein 115 (RNF115) is an E3 ligase implicated in tumorigenesis and metastasis.
- Its specific role and mechanism in thyroid carcinoma (THCA) progression remain to be fully elucidated.
Purpose of the Study:
- To investigate the function and mechanism of RNF115 in the development of THCA.
- To identify downstream targets and signaling pathways regulated by RNF115 in THCA.
Main Methods:
- RNF115 expression analysis in THCA using TCGA database.
- In vitro and in vivo experiments to assess RNF115's impact on THCA progression.
- Bioinformatics analysis to identify RNF115-regulated proteins and pathways.
Main Results:
- RNF115 expression is upregulated in THCA tissues and cells.
- Elevated RNF115 promotes THCA cell proliferation, tumor growth, epithelial-mesenchymal transition (EMT), and lung metastasis.
- CDK10 identified as a downstream target, ubiquitinated and degraded by RNF115, counteracting RNF115's pro-tumorigenic effects.
- RNF115 activates the Raf-1 pathway and enhances cell cycle progression via CDK10 degradation.
Conclusions:
- RNF115 drives THCA proliferation, EMT, and metastasis through ubiquitinating and degrading CDK10.
- This mechanism involves the activation of the Raf-1 pathway and altered cell cycle progression in THCA.
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