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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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LanTERN: A Fluorescent Sensor That Specifically Responds to Lanthanides.

Ethan M Jones1,2, Yang Su1, Chris Sander1

  • 1Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, United States.

ACS Synthetic Biology
|February 20, 2024
PubMed
Summary

Researchers developed LanTERN, a novel lanthanide-responsive fluorescent protein, to overcome limitations in measuring lanthanide binding. This breakthrough enables new biosensing systems for these critical elements.

Keywords:
EF handGCaMPLanMlanmodulinlanthaniderare-earth element

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Area of Science:

  • Biochemistry
  • Synthetic Biology
  • Molecular Biology

Background:

  • Lanthanides are essential in modern technology but their purification is environmentally costly.
  • Current biochemical tools are insufficient for measuring lanthanide binding, hindering synthetic biology advancements.

Purpose of the Study:

  • To develop a novel biosensor for detecting lanthanide ions.
  • To engineer a lanthanide-responsive fluorescent protein for improved detection.

Main Methods:

  • Engineered mutations in a calcium indicator (GCaMP) to alter ion specificity.
  • Utilized EF hand motifs for lanthanide binding.
  • Measured fluorescence changes in response to lanthanide binding.

Main Results:

  • Developed LanTERN, a fluorescent protein responsive to lanthanides.
  • LanTERN exhibits a 14-fold or greater increase in fluorescence upon binding 10 different lanthanides.
  • Successfully switched ion specificity from calcium to lanthanides.

Conclusions:

  • LanTERN provides a new tool for measuring lanthanide ions.
  • This development facilitates the creation of advanced lanthanide-binding proteins and biosensing systems.
  • Offers a more sustainable approach to lanthanide detection and utilization.