Comparison of extraction processes, characterization and intestinal protection activity of Bletilla striata polysaccharides

Junjie Qiu ¹, Xiao Xu ², Jingyan Guo ¹

⁰School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.

International Journal of Biological Macromolecules +

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February 20, 2024

View abstract on PubMed

Summary

Optimized Bletilla striata polysaccharide extraction using Box-Behnken design and genetic algorithm-back propagation improved yield and antioxidant activity. The purified polysaccharide demonstrated intestine protective effects in cell models.

Area Of Science

Biochemistry
Pharmacology
Materials Science

Background

Bletilla striata polysaccharides (BSP) are known for their bioactivities.
Optimizing extraction methods is crucial for maximizing BSP yield and efficacy.
Understanding the structural and functional properties of BSP is essential for its applications.

Purpose Of The Study

To optimize the extraction of Bletilla striata polysaccharides (BSP) using orthogonal design, Box-Behnken design (BBD), and genetic algorithm-back propagation (GA-BP).
To characterize the structure and properties of the purified BSP (pBSP1).
To evaluate the protective effects of pBSP1 on intestinal cells.

Main Methods

Extraction process optimization using orthogonal design, BBD, and GA-BP.
Purification of BSP to obtain pBSP1.
Structural characterization using SEM, UV, FTIR, HPLC, XRD, NMR, and periodate oxidation.
Assessment of thermal stability and viscoelastic behavior.
In vitro evaluation of protective effects on LPS-induced GES-1 and Caco2 cells.

Main Results

The optimal extraction parameters (liquid-to-solid ratio 15 mL/g, power 450 W, time 34 min) yielded 8.29% BSP with high antioxidant activity.
Purified pBSP1 (Mw 255.172 kDa) exhibited an amorphous structure with specific glucose and mannose compositions and good thermal/viscoelastic properties.
pBSP1 reduced apoptosis, reactive oxygen species (ROS) production, and lowered transepithelial electrical resistance (TEER) synthesis in intestinal cells.

Conclusions

The combination of BBD and GA-BP effectively optimized BSP extraction, enhancing yield and antioxidant activity.
pBSP1 possesses a unique structure and favorable physicochemical properties.
pBSP1 demonstrates potential as an intestine-protective agent by mitigating LPS-induced cellular damage.

Keywords:

Characterization Extraction Intestinal protection