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The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium

S M Niedziółka1,2, S Datta1, T Uśpieński1,2

  • 1Centre of New Technologies, University of Warsaw, Warsaw, Poland.

Communications Biology
|February 20, 2024
PubMed

Insights

The exocyst complex facilitates soluble protein transport into primary cilia via endosome recycling. This study identifies key GTPases involved in trafficking crucial for cell signaling and development.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Efficient protein transport into primary cilia is vital for signaling pathways.
  • Cilium dysfunction is linked to developmental disorders and cancer.
  • Mechanisms for soluble protein ciliary import remain largely unknown.

Purpose of the Study:

  • To investigate the mechanisms of soluble protein transport into primary cilia.
  • To identify key protein complexes and GTPases involved in this process.

Main Methods:

  • Utilized genetic and cell biological approaches.
  • Investigated the role of the exocyst complex in ciliary trafficking.
  • Analyzed the involvement of endosome recycling machinery and specific GTPases.

Main Results:

  • Identified the exocyst complex as essential for ciliary trafficking of soluble Gli transcription factors.
  • Demonstrated that soluble proteins use endosome recycling pathways for ciliary import.
  • Pinpointed Rab14, Rab18, Rab23, and Arf4 as critical GTPases in vesicle-mediated Gli transport.

Conclusions:

  • The exocyst complex and endosome recycling machinery mediate soluble protein import into primary cilia.
  • Specific GTPases regulate vesicle-mediated transport of Gli proteins to cilia.
  • These findings enhance understanding of ciliary transport and intracellular mechanisms.

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