From 2D slices to a 3D model: Training students in digital microanatomy analysis techniques through a 3D printed neuron project

Jordan M Renna ¹, Katelyn B Sondereker ¹, Christopher L Cors ¹

⁰Department of Biology, University of Akron, Akron, Ohio, USA.

Anatomical Sciences Education +

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February 21, 2024

View abstract on PubMed

Summary

This study introduces a new activity for students to create 3D neuron models from 2D images. This hands-on approach enhances understanding of neuroanatomy and image analysis skills.

Area Of Science

Neuroscience Education
Digital Anatomy
3D Modeling

Background

Modern anatomy education increasingly requires 3D digital modeling skills, yet these are seldom taught in undergraduate curricula.
There is a growing need for learning activities that integrate 2D and 3D anatomical exploration.
Current methods for teaching microanatomy and neuroanatomy may not fully leverage digital tools.

Purpose Of The Study

To present a novel, accessible learning activity for students to construct 3D digital anatomical models from serial 2D image data.
To enhance students' comprehension of neuron morphology and develop technical image analysis skills.
To provide a practical workflow for 3D cell modeling applicable to broader microanatomy and neuroanatomy education.

Main Methods

Students digitally trace neurons from confocal microscopy image stacks.
A 3D digital model of the neuron is created from the traced 2D slices.
The 3D model is prepared for 3D printing, allowing for tactile exploration.

Main Results

The learning activity successfully engages students in producing a tangible 3D digital model of a neuron.
Students gain didactic knowledge of neuron morphology and practical image analysis skills.
The workflow is demonstrated to be inexpensive, achievable, and adaptable for various cell types.

Conclusions

This 3D modeling activity offers a unique method for students to interact with microanatomy, bridging 2D imaging and 3D visualization.
The described workflow serves as a proof of principle for integrating 3D printing into anatomy education.
The approach aims to stimulate the adoption of 3D printing technologies in neuroanatomy and broader microanatomy instruction.

Keywords:

3D modeling 3D printing digital morphology microanatomy neuroanatomy neuron