Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A quantitative proteomics dataset for assessment and prediction of low dose X-ray radiation exposure in mice.

bioRxiv : the preprint server for biology·2026

Same author

Accuracy comparison of 4D computed tomography (4DCT) and 4D cone beam computed tomography (4DCBCT).

International journal of medical physics, clinical engineering and radiation oncology·2026

Same author

Split-Dose FLASH Irradiation to Investigate the Clinical Feasibility of Multifield Treatments: The Effect of Split Dose and Dose Rate on FLASH.

International journal of radiation oncology, biology, physics·2026

Same author

PTCOG Ocular Survey - Perspective on Ocular Particle Therapy: Current Practices and Emerging Trends.

International journal of particle therapy·2026

Same author

Quantifying cervical cancer radiotherapy care gap: Baseline assessment prior to implementation of a digital Health App.

Technical innovations & patient support in radiation oncology·2026

Same author

Initiating Proton Ocular Treatments on General-Purpose Beamlines: Challenges and Trends From the Survey of US Facilities.

International journal of radiation oncology, biology, physics·2025

Search research articles

Related Experiment Video

Updated: Jul 2, 2025

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

Published on: March 24, 2022

Preclinical Ultra-High Dose Rate (FLASH) Proton Radiation Therapy System for Small Animal Studies.

Ning Cao¹, Danielle P Johnson Erickson¹, Eric C Ford¹

¹Department of Radiation Oncology, University of Washington School of Medicine, Seattle, Washington.

Advances in Radiation Oncology

|February 21, 2024

Summary

Ultrahigh dose-rate radiation therapy (FLASH) significantly improved survival in preclinical models compared to conventional dose-rate therapy. FLASH spares normal tissues while maintaining tumor control, offering a promising advancement in radiation oncology.

More Related Videos

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

Published on: December 28, 2017

Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies

Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies

Published on: February 6, 2019

Related Experiment Videos

Last Updated: Jul 2, 2025

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform

Published on: March 24, 2022

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

Published on: December 28, 2017

Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies

Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies

Published on: February 6, 2019

Area of Science:

Radiation Oncology
Preclinical Research
Medical Physics

Background:

Ultrahigh dose-rate radiation therapy (FLASH) shows potential for sparing normal tissues while maintaining antitumor efficacy compared to conventional dose-rate radiation therapy (CONV).
A preclinical scattered proton beam system operating at FLASH dose rates has been developed to investigate these effects.

Purpose of the Study:

To present the technical details of a novel preclinical FLASH proton radiation system.
To evaluate the preliminary biological outcomes of whole pelvis radiation using this system in a preclinical model.

Main Methods:

A modified Scanditronix MC50 cyclotron produced a 48.7 MeV proton beam with dose rates ranging from 0.1 to 150 Gy/s.
Female C57BL/6 mice underwent whole pelvis irradiation (18.5 Gy) at CONV (0.6-1 Gy/s) or FLASH (50-80 Gy/s) dose rates to assess normal tissue effects (survival, proliferation, apoptosis).
Tumor control was evaluated in B16F10 allografts receiving 18 Gy CONV or FLASH proton radiation.

Main Results:

Whole pelvis irradiation resulted in 100% survival in controls, 0% in CONV, and 44% in FLASH groups (P < .01).
FLASH irradiation led to significantly higher distal colon cell proliferation (EdU staining) and lower apoptosis (cleaved caspase-3 staining) compared to CONV.
Tumor control was comparable between CONV and FLASH radiation groups.

Conclusions:

The developed preclinical FLASH proton radiation system demonstrates improved normal tissue sparing, evidenced by enhanced survival and reduced apoptosis.
FLASH radiation achieved equivalent tumor control to CONV, suggesting its potential as a superior therapeutic option in radiation oncology.