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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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The technique...
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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Related Experiment Video

Updated: Jul 2, 2025

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression
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Exploring Circular RNA Profile and Expression in Extracellular Vesicles.

Jingjing Zhao1, Qiaojuan Li1, Shenglin Huang2

  • 1Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2024
PubMed
Summary

Investigating circular RNA (circRNA) expression within extracellular vesicles (EVs) offers insights into disease pathology. A described workflow aids in analyzing these EV-circRNAs for diagnostic and therapeutic potential.

Keywords:
ASJABioinformaticsCIRI2Circular RNAExtracellular vesiclesRNA isolation

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Extracellular vesicles (EVs) are secreted by cells and found in body fluids.
  • EVs carry RNA, influencing biological processes and holding diagnostic/therapeutic potential.
  • Circular RNAs (circRNAs), formed by back-splicing, are abundant in EVs and implicated in disease.

Purpose of the Study:

  • To present a comprehensive workflow for analyzing the expression profile of circRNAs within EVs.
  • To facilitate research into the role of EV-circRNAs in disease pathology.

Main Methods:

  • EV separation from biological fluids.
  • RNA library preparation for sequencing.
  • Bioinformatics analysis of circRNA expression data.

Main Results:

  • The workflow enables detailed investigation of EV-circRNA expression profiles.
  • Identified circRNAs can be correlated with specific disease states.

Conclusions:

  • The presented workflow is crucial for understanding EV-circRNA functions.
  • This research aids in exploring EV-circRNAs for disease biomarker and therapeutic target discovery.