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RNAi Interference by dsRNA Injection into Drosophila Embryos
Published on: April 11, 2011
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In Vivo Tissue-Specific Knockdown of circRNAs Using shRNAs in Drosophila melanogaster
Ines Lucia Patop1, Michael Canori1, Sebastian Kadener2
1Brandeis University, Waltham, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2024
Abstract
None:
Studying circular RNAs' function in vivo has been challenging due to the lack of generic tools to manipulate their levels without affecting their linear counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdown with tissue-specific resolution in flies.
