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Meiotic Spindle View improves the Outcome of IVF in Poor Responders: A Retrospective Analytical Study from an

Binarwan Halim1, Hilma Putri Lubis1, Ichwanul Adenin1

  • 1HFC IVF center Division of Reproductive, Endocrinology and Infertility Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia.

JBRA Assisted Reproduction
|February 21, 2024
PubMed
Abstract

Insights

Viewing the meiotic spindle during in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) may improve embryo cleavage rates in poor responder women. This technique helps optimize IVF outcomes for challenging cases.

Area of Science:

  • Reproductive biology
  • Assisted reproductive technology
  • Oocyte maturation

Background:

  • The meiotic spindle in human oocytes is not always aligned with the first polar body.
  • Polarized Light Microscopy (PLM) allows visualization of the meiotic spindle without disruption.
  • Oocyte spindle positioning is crucial for successful fertilization and embryo development.

Purpose of the Study:

  • To investigate the impact of meiotic spindle viewing (MSV) on IVF-ICSI outcomes.
  • To evaluate the relationship between spindle alignment and success rates in poor responder women undergoing IVF-ICSI.

Main Methods:

  • Retrospective analysis of 115 poor responder women undergoing IVF-ICSI.
  • Patients divided into two groups: non-MSV (control) and MSV (case).
  • Meiotic spindles imaged using Oosight microscopy before ICSI.

Main Results:

  • No significant difference in fertilization rates between MSV and non-MSV groups.
  • Significantly higher embryo cleavage rates in the MSV group compared to the non-MSV group.
  • Higher fertilization rates observed with aligned spindles; improved good quality embryo rates in the MSV group.

Conclusions:

  • Meiotic spindle viewing may enhance IVF outcomes in poor responder women.
  • Spindle visualization is a potentially valuable tool for improving IVF success.
  • Further research is warranted to confirm the benefits of MSV in assisted reproduction.
Keywords:
IVFmeiotic spindle viewoocytepoor responder

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