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Updated: Jul 2, 2025
Cholesterol Efflux Assay
Published on: March 6, 2012
Impaired Cholesterol Efflux Capacity rather than Low HDL-C Reflects Oxidative Stress under Acute Myocardial
Kentaro Oniki1, Masatsune Ogura2,3, Erika Matsumoto1
1Division of Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Kumamoto University.
Insights
Cholesterol efflux capacity (CEC), not HDL-C levels, reflects oxidative stress in acute myocardial infarction (AMI) patients. CEC measurement offers insights into systemic oxidative stress and atherosclerosis risk for AMI patients.
Area of Science:
- Cardiovascular Medicine
- Biochemistry
- Oxidative Stress Research
Background:
- Acute myocardial infarction (AMI) causes irreversible heart damage and systemic oxidative stress.
- High-density lipoprotein (HDL) possesses antioxidant properties, with reduced capacity linked to decreased cholesterol efflux capacity (CEC).
Purpose of the Study:
- To evaluate the clinical utility of measuring CEC in patients diagnosed with AMI.
- To investigate the relationship between CEC and systemic oxidative stress markers in AMI.
Main Methods:
- A case-control study involving 193 AMI patients and 445 controls.
- Examined associations between CEC, HDL-cholesterol (HDL-C), and oxidized human serum albumin (HSA) as an oxidative stress marker.
- Assessed the influence of ALDH2 rs671 polymorphism on these associations.
Main Results:
- CEC correlated positively with HDL-C in both AMI cases and controls, though weaker in AMI.
- In AMI patients, CEC was associated with oxidized HSA levels, but HDL-C was not.
- No significant differences in these associations were observed across ALDH2 genotypes.
Conclusions:
- CEC, unlike HDL-C levels, serves as a reliable indicator of systemic oxidative stress in AMI patients.
- CEC measurement provides valuable information regarding systemic oxidative stress and atherosclerosis risk in AMI.
Aims:
Acute myocardial infarction (AMI) causes irreversible damage to cardiomyocytes due to the discontinuation of oxygen supply and leads to systemic oxidative stress. It has been reported that high-density lipoprotein (HDL) particles have antioxidant capacity, and reduced antioxidant capacity is associated with decreased cholesterol efflux capacity (CEC). The purpose of this study was to clarify the usefulness of CEC measurement in patients with AMI.
Methods:
We investigated the association between CEC and oxidative stress status in a case-control study. This study included 193 AMI cases and 445 age- and sex-matched controls. We examined the associations of CEC with HDL-cholesterol (HDL-C) and oxidized human serum albumin (HSA), an index of systemic oxidative stress status, and the effect of aldehyde dehydrogenase 2 (ALDH2) rs671 polymorphism, which has been reported to affect HDL-C level and risk for MI, on these associations.
Results:
Both bivariable and multivariable analyses showed that CEC was positively correlated with HDL-C levels in both AMI cases and controls, with a weaker correlation in AMI cases than in controls. In AMI cases, oxidized HSA levels were associated with CEC in both bivariable and multivariable analyses, but not with HDL-C. These associations did not differ among the ALDH2 genotypes.
Conclusions:
CEC, but not HDL-C level, reflects systemic oxidative stress status in patients with AMI. CEC measurement for patients with AMI may be useful in that it provides information on systemic oxidative stress status as well as atherosclerosis risk.
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