Prognosis and biological function of SGOL1 in clear cell renal cell carcinoma: a multiomics analysis

Zezhong Yang ¹, Yunzhong Jiang ¹, Lu Wang ¹

⁰Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Address: No.277 Yanta West Road, Xi'an, Shaanxi, 710061, China.

Bmc Medical Genomics +

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February 21, 2024

View abstract on PubMed

Summary

Shugoshin-1 (SGOL1) is highly expressed in clear cell renal cell carcinoma (ccRCC), promoting tumor progression and potentially serving as a therapeutic target. This study explores SGOL1

Area Of Science

Oncology
Molecular Biology
Genetics

Background

Shugoshin-1 (SGOL1) is crucial for chromosome segregation and its aberrant expression correlates with tumor progression.
The role and expression of SGOL1 in clear cell renal cell carcinoma (ccRCC) remain largely uncharacterized.

Purpose Of The Study

To investigate the expression pattern, biological function, and prognostic significance of SGOL1 in ccRCC.
To explore the association of SGOL1 with the tumor microenvironment (TME) and identify potential regulatory axes in ccRCC.

Main Methods

Utilized TCGA and GEO databases for mRNA expression and outcome data; validated protein expression via immunohistochemistry (IHC).
Employed bioinformatics tools (UALCAN, TISIDB, TIMER, etc.) for data analysis, functional enrichment (GO, GSEA), and immune infiltration assessment.
Conducted in vitro assays (MTT, EdU, flow cytometry, Transwell, wound healing) to determine SGOL1's functional role in ccRCC cells.

Main Results

SGOL1 was significantly upregulated in ccRCC, correlating with adverse clinicopathological features and poor prognosis.
SGOL1 is implicated in cell cycle regulation, p53 pathway, DNA replication, and T-cell activation, and is associated with increased Treg infiltration and immune checkpoint expression.
A novel regulatory axis (SNHG17/PVT1/ZMIZ1-AS1-miR-23b-3p-SGOL1) was identified, and SGOL1 was shown to promote ccRCC cell proliferation, migration, and invasion.

Conclusions

SGOL1 acts as an oncogene in ccRCC, driving tumor progression and contributing to an immunosuppressive TME.
SGOL1 may serve as an independent prognostic biomarker for ccRCC.
Targeting SGOL1 presents a potential novel therapeutic strategy for ccRCC treatment.

Keywords:

Immune infiltration Malignant progression Prognostic biomarkers SGOL1 ccRCC

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