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Micrometastasis detection using modified papanicolaou stain in nodal tissues of oral squamous cell carcinoma - A histological study

  • 0Department of Oral Pathology and Microbiology, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.
Journal of Cancer Research and Therapeutics +

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February 22, 2024

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February 22, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Modified Papanicolaou (PAP) stain accurately detects micrometastasis in oral squamous cell carcinoma (OSCC) lymph nodes. This improved detection aids in precise staging and treatment planning for SCCHN patients.

Area Of Science

  • Oncology
  • Pathology
  • Surgical Pathology

Background

  • Cervical lymph node metastasis is a critical prognostic factor in Head and Neck Squamous Cell Carcinoma (HNSCC).
  • Accurate detection of micrometastasis is vital for staging, treatment, and prognosis in HNSCC.
  • Assessing lymph node metastasis without clinical enlargement presents diagnostic challenges.

Purpose Of The Study

  • To evaluate micrometastasis and individual tumor cells (ITC) in regional lymph nodes of oral squamous cell carcinoma (OSCC).
  • To assess the efficacy of modified Papanicolaou (PAP) stain compared to H&E stain for micrometastasis detection.
  • To re-evaluate tumor staging based on enhanced micrometastasis detection.

Main Methods

  • Retrospective study involving 40 lymph nodes from OSCC patients (20 metastatic, 20 non-metastatic).
  • Histopathological analysis using Hematoxylin and Eosin (H&E) stain followed by modified Papanicolaou (PAP) stain.
  • Statistical analysis using the Chi-square test to determine significance.

Main Results

  • Modified PAP stain demonstrated significantly higher accuracy (p = 0.006) than H&E stain in identifying micrometastasis.
  • Micrometastasis was detected in 15% of lymph node sections initially classified as non-metastatic by H&E.
  • The study highlights the sensitivity of modified PAP stain for micrometastasis detection.

Conclusions

  • Modified PAP stain is a valuable and sensitive tool for detecting micrometastasis in OSCC lymph nodes, outperforming H&E.
  • Detecting micrometastasis in OSCC is clinically advantageous, influencing patient staging.
  • Identification of micrometastasis impacts treatment strategies, including radiotherapy and chemotherapy regimens.