Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimized chemogenetic ablation and regeneration of enteric nervous system neurons in zebrafish.

Stem cell reports·2026
Same author

Suppression of Ciliogenesis Alleviates Cellular Senescence via AKT Signaling in Gingival Aging.

Aging cell·2026
Same author

Self-vocalizations activate the developing auditory cortex via an intracortical pathway.

Science advances·2026
Same author

Optimization of spatial and temporal sampling resolution for optophysiology in large-scale two-photon calcium imaging.

Biomedical optics express·2026
Same author

Fluorescently-labeled split-QF hemidrivers: simplifying and enhancing methods enabling intersectional targeting of discrete cell types.

bioRxiv : the preprint server for biology·2026
Same author

Layer 6 corticothalamic neurons show diverse and dynamic responses that support a role in cortical gain control in noisy environments.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jul 2, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

2.0K

Mesoscopic oblique plane microscopy with a diffractive light-sheet for large-scale 4D cellular resolution imaging.

Wenjun Shao1,2, Minzi Chang1, Kevin Emmerich1

  • 1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21231, USA.

Optica
|February 22, 2024
PubMed
Summary

Mesoscopic oblique plane microscopy (Meso-OPM) offers a sixfold axial resolution improvement for large-scale 3D imaging. This breakthrough enables whole-organism visualization of dynamic cellular processes in vivo at unprecedented speeds.

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.8K
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

8.0K

Related Experiment Videos

Last Updated: Jul 2, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

2.0K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.8K
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

8.0K

Area of Science:

  • Biomedical Imaging
  • Microscopy Technology
  • Neuroscience

Background:

  • Observing dynamic biological processes in 3D across mesoscopic scales requires high-resolution, large-field-of-view imaging.
  • Existing microscopy techniques often face trade-offs between field of view and axial resolution, limiting the study of rapid, whole-organism phenomena.

Purpose of the Study:

  • To develop a novel microscopy technique, mesoscopic oblique plane microscopy (Meso-OPM), to overcome current FOV and resolution limitations.
  • To achieve high-speed, 3D volumetric imaging of biological structures at cellular resolution across mesoscopic scales.

Main Methods:

  • Development of Meso-OPM utilizing a diffractive light sheet with an augmented illumination angle via a transmission grating.
  • Demonstration of enhanced axial resolution, approximately sixfold improvement over existing methods.
  • Achieved a large field of view (5.4 mm × 3.3 mm) with high resolution (2.5 μm × 3 μm × 6 μm) for single-scan volumetric imaging.

Main Results:

  • Meso-OPM achieved approximately sixfold improvement in axial resolution.
  • Demonstrated volumetric imaging of 3D cellular structures within a large FOV (5.4 mm × 3.3 mm).
  • Successfully performed in vivo whole-body volumetric recordings of neuronal activity at 2 Hz and blood flow dynamics at 5 Hz in zebrafish larvae with 3D cellular resolution.

Conclusions:

  • Meso-OPM provides a powerful tool for large-scale, high-speed, 3D dynamic imaging in biological systems.
  • This technique enables unprecedented insights into whole-organism physiological processes at cellular resolution.
  • Meso-OPM overcomes critical limitations in current microscopy, advancing the study of complex biological systems.