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The H-2 histocompatibility system and lymphocyte adhesion: interaction modulation factor involvement.

A S Curtis

    Journal of Immunogenetics
    |June 1, 1979
    PubMed
    Summary
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    Thymocyte interaction modulation factors (IMF) reduce cell adhesion. Allogeneic T-IMFs diminish thymocyte and B-cell adhesion, particularly when mismatched at the H-2 D locus, aiding cell typing.

    Area of Science:

    • Immunology
    • Cell Biology
    • Genetics

    Background:

    • Thymocyte interaction modulation factor (IMF) is a glycoprotein produced by thymocytes.
    • IMF has been shown to reduce the adhesion of syngeneic B-cells, leucocytes, and macrophages.
    • Cell adhesion plays a crucial role in various biological processes, including immune responses.

    Purpose of the Study:

    • To investigate the allogeneic effects of thymocyte IMF on cell adhesion.
    • To determine the role of the H-2 D locus in IMF-mediated adhesion modulation.
    • To explore the potential of anti-IMF antibodies for cell typing and understanding IMF localization.

    Main Methods:

    • Collision efficiency method to measure cell adhesion in suspension culture.
    • Preparation of thymocyte IMFs from different congenic mouse strains.

    Related Experiment Videos

  • Testing IMFs on syngeneic and allogeneic thymocytes and B-cells.
  • Immunofluorescence and immune cytolysis assays using antibodies against thymocyte IMFs.

    • Main Results:

    • Allogeneic T-IMFs diminish thymocyte and B-cell adhesion more than syngeneic T-IMFs.
    • Mismatches at the H-2 D locus between IMF and cells significantly reduce cell adhesion.
    • Anti-IMF antibodies can detect T-IMF on cell surfaces and are useful for H-2 D typing.
    • Thymocyte IMF is associated with the H-2 D locus and present on thymocytes and other cell types.

    Conclusions:

    • Thymocyte IMF exhibits both syngeneic and allogeneic effects on cell adhesion.
    • The H-2 D locus is critical for the specific binding and action of thymocyte IMF.
    • Anti-IMF antibodies provide a novel tool for cell typing and understanding cell recognition mechanisms.