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Updated: Jul 2, 2025

3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii

3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii

Published on: December 9, 2014

Advancing Toxoplasma gondii multiplex serology.

Rima Jeske¹, Nico Becker^1,2, Lea Kroeller^1,3

¹Division of Infections and Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Microbiology Spectrum

|February 22, 2024

Summary

This study improved a multiplex serology assay for Toxoplasma gondii (T. gondii) detection. The enhanced assay provides accurate and reproducible antibody measurements, crucial for large-scale seroprevalence studies and understanding T. gondii infection.

Keywords:

Toxoplasma gondii high-throughput serology multiplex serology public health seroepidemiology

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Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

Area of Science:

Immunology
Infectious Diseases
Public Health

Background:

Toxoplasma gondii is a prevalent zoonotic pathogen with significant public health implications.
Current understanding of long-term consequences, risk factors, and co-infections related to T. gondii remains limited.
Large-scale seroepidemiological studies are essential for addressing these knowledge gaps but require robust diagnostic tools.

Purpose of the Study:

To significantly advance a previously developed multiplex serology assay for T. gondii.
To improve assay characteristics for high-throughput, large-scale seroprevalence studies.
To enable reliable detection of T. gondii antibodies under diverse assay conditions.

Main Methods:

Development of truncated versions of immunodominant antigens (SAG1D1 and P22trunc) for enhanced assay performance.
Evaluation of assay performance using magnetic and non-magnetic beads at various sample dilutions (1:100 and 1:1,000).
Comparison with the gold-standard Sabin-Feldman dye test and derivation of seropositivity thresholds using finite mixture models and ROC analysis.

Main Results:

The improved assay using SAG1D1 and P22trunc demonstrated significantly enhanced signal-to-noise ratios and near-perfect concordance with the Sabin-Feldman dye test.
High diagnostic accuracy was achieved at 1:100 dilution (Sensitivity: 98% for SAG1D1, 94% for P22trunc; Specificity: 93% for SAG1D1, 95% for P22trunc).
Reproducible performance metrics were confirmed at 1:1,000 dilution with both magnetic and non-magnetic beads.

Conclusions:

The revised multiplex serology assay provides robust and reproducible T. gondii antibody measurements across various conditions.
This advancement facilitates the inclusion of T. gondii detection in multi-pathogen multiplex serology panels for large-scale research.
The improved assay is a valuable tool for enhancing insights into T. gondii epidemiology, risk factors, and public health impact.