Diagnostic value of long noncoding RNA SNHG15 in gastric cancer: in vitro and in silico studies

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Long non-coding RNA SNHG15 is implicated in various cancers.
• The precise role and diagnostic value of SNHG15 in gastric cancer (GC) remain unclear.

Purpose of the Study:

• To investigate the diagnostic potential of SNHG15 in GC patients.
• To elucidate the role of the SNHG15-miRNA-mRNA network in GC pathobiology.

Main Methods:

• Quantitative real-time PCR (qRT-PCR) to assess SNHG15 expression in GC tissues and adjacent normal tissues (ANTs).
• Correlation analysis with clinicopathological factors.
• Receiver Operating Characteristic (ROC) curve analysis for diagnostic evaluation.
• Gene Ontology (GO) and KEGG pathway analysis for network investigation.

Main Results:

• SNHG15 was significantly overexpressed in GC tissues compared to ANTs (fold change = 3.87, P = 0.0022).
• SNHG15 expression did not show significant association with clinicopathological factors.
• ROC analysis demonstrated diagnostic potential with AUC = 0.744 (P < 0.0001), sensitivity = 79.73%, and specificity = 63.51%.
• Network analysis identified interactions between SNHG15, has-miR-613, has-miR-542-3p, and has-miR-1236-3p, potentially affecting EGFR tyrosine kinase inhibitor resistance, HIF-1, and VEGF signaling pathways.

Conclusions:

• SNHG15 may serve as a valuable diagnostic biomarker for gastric cancer.
• The SNHG15-miRNA-mRNA network is likely involved in GC progression through key cancer-related signaling pathways.