Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.6K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial Intelligence and Detection of Hirschsprung Disease.

The New England journal of medicine·2026
Same author

Editorial: New insights on B cell development in poultry.

Frontiers in immunology·2026
Same author

Functional model for amelogenesis: polarization and pH sensitivity of calcium uptake in ameloblast-derived HAT-7 cells.

Calcified tissue international·2026
Same author

Development and Formulation of Nanofiber-Based Ophthalmic Inserts for the Treatment of Fungal Keratitis.

Pharmaceutics·2026
Same author

Duodenal hematoma following endoscopic duodenal biopsy in an adult requiring arterial embolization and surgical evacuation: a case report and review of the literature.

Frontiers in gastroenterology (Lausanne, Switzerland)·2026
Same author

Glial Cell Line-derived Neurotrophic Factor and Retinoic Acid Synergy Unlocks Neurogenesis in Adult Myenteric Glia/Neural Progenitors.

Cellular and molecular gastroenterology and hepatology·2026

Related Experiment Video

Updated: Jul 2, 2025

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
08:26

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

9.3K

Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation.

Jessica L Mueller1, Rhian Stavely1, Richard A Guyer1

  • 1Department of Pediatric Surgery, Massachusetts General Hospital, Boston, MA, USA.

Stem Cells Translational Medicine
|February 22, 2024
PubMed
Summary

Inhibiting agrin, an extracellular matrix protein, enhances the migration of transplanted cells for Hirschsprung disease. This finding improves potential regenerative cell therapies for the condition.

Keywords:
Hirschsprung diseaseenteric nervous systementeric neuronal stem cellsextracellular matrixstem cell therapy

More Related Videos

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
07:53

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Published on: August 15, 2018

13.1K
Immunostaining to Visualize Murine Enteric Nervous System Development
07:54

Immunostaining to Visualize Murine Enteric Nervous System Development

Published on: April 29, 2015

11.4K

Related Experiment Videos

Last Updated: Jul 2, 2025

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
08:26

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

9.3K
Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
07:53

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Published on: August 15, 2018

13.1K
Immunostaining to Visualize Murine Enteric Nervous System Development
07:54

Immunostaining to Visualize Murine Enteric Nervous System Development

Published on: April 29, 2015

11.4K

Area of Science:

  • Regenerative medicine
  • Developmental biology
  • Gastroenterology

Background:

  • Hirschsprung disease (HSCR) treatment faces challenges due to poor transplanted cell migration in the postnatal gut.
  • The extracellular environment significantly impacts enteric neural crest-derived cell (ENCDC) migration during nervous system development.

Purpose of the Study:

  • To identify factors hindering cell migration in HSCR and develop strategies to improve cell engraftment.
  • To compare gene expression in embryonic and postnatal ENCDCs to understand migration differences.

Main Methods:

  • Transcriptomic analysis of embryonic and postnatal ENCDCs to identify differentially expressed extracellular matrix (ECM) genes.
  • Investigated the role of agrin, a highly expressed ECM protein in postnatal cells, using function-blocking antibodies and shRNA.
  • Assessed ENCDC migration in vitro, ex vivo, and in vivo following agrin inhibition.

Main Results:

  • Identified 89 differentially expressed ECM-associated genes between embryonic and postnatal ENCDCs.
  • Agrin was significantly over-expressed in postnatal cells and inhibited ENCDC migration.
  • Inhibiting agrin promoted ENCDC migration, particularly GFAP+ cells, in various experimental models.

Conclusions:

  • Agrin is a key factor inhibiting ENCDC migration in the postnatal gut environment.
  • Targeting agrin can enhance cell migration, offering a promising strategy for regenerative cell therapies in Hirschsprung disease.
  • Modulating the extracellular environment is crucial for successful stem cell transplantation in HSCR.