Effects of expeller soybean on growth performance, amino acid digestibility and intestinal integrity

Expeller soybean (ESB) is a widely used protein source in broiler diets due to its high amino acid digestibility. However, improper heat processing of ESB can negatively affect nutrient digestion, absorption, and metabolism leading to decreased growth performance. The study aimed to investigate the impact of varying processing temperatures on growth performance, amino acid digestibility (AID), and intestinal integrity using 3 different commercial batches of ESB processed at distinct temperatures. These temperatures were 182°C (normal-control), 199°C (overcooked), and 154°C (undercooked). 1,860 off-sex male Cobb 500 broilers were allocated randomly to these treatments, with 10 replicate floor pens (62 birds/pen) from 1 to 35 d of age. Birds consuming the overcooked ESB exhibited significantly lower body weight gain (BWG) and feed intake (FI) on d 14, 28, and 35. They also showed higher feed conversion ratio (FCR) and smaller relative right pectoralis major (RPM) weights at d 35. Meanwhile, birds fed undercooked ESB demonstrated reduced BWG at d 14. Serum fluorescein isothiocyanate-dextran (FITC-d; 4 kD) concentrations on d 16 were notably elevated in birds fed overcooked ESB, indicating increased gut permeability. Overcooked ESB reduced the AID coefficients of several amino acids on d 14 and 28, with Lys experiencing the highest reduction (8%). Undercooked ESB, however, mainly affected the AID of Val, and Phe at d 28. In conclusion, overcooked ESB decreased amino acid digestibility, impaired gut barrier function, and led to diminished growth performance. Conversely, undercooked ESB primarily affected the digestibility of Val and Phe and resulted in reduced BWG at d 14. These findings underscore the critical role of proper heat processing in preserving the nutritional quality of ESB in broiler diets, influencing optimal growth performance, and maintaining intestinal health.