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Preparation and characterization of tilapia protein isolate - Hyaluronic acid complexes using a pH-driven method for
Mengya Xie1, Chunxia Zhou2, Xiang Li1
1College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China; Guangdong Provincial Engineering Technology Research Center of Marine Food, Zhanjiang 524088, China; Guangdong Provincial Modern Agricultural Science and Technology Innovation Center, Zhanjiang 524088, China.
Abstract:
This study aimed to investigate the non-covalent complexation between hyaluronic acid (HA) and tilapia protein isolate (TPI) on the stability of oil-in-water (O/W) TPI emulsion. The results showed that HA binds to TPI through electrostatic, hydrophobic, and hydrogen bonding interactions, forming homogeneous hydrophilic TPI-HA complexes. The binding of HA promoted the structural folding of TPI and altered its secondary structure during pH neutralization. The TPI-HA complexes presented significantly improved EAI and ESI (P < 0.05) when the HA concentration was 0.8 % (w/v). Emulsion characterization showed that HA promoted the transfer of TPI to the O/W interface, forming an emulsion with excellent stability, which, combined with the high surface charge and strong spatial site resistance effect of HA, improved TPI emulsion stability. Therefore, non-covalent complexation with HA is an effective strategy to improve the stability of TPI emulsion.
