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Related Experiment Video

Updated: Jul 2, 2025

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

A top variant identification pipeline for protein engineering.

Hui Chen¹, Zhike Lu², Lijia Ma³

¹Westlake Genetech, Hangzhou, China.

|February 22, 2024

Summary

This study introduces TopVIP, a pipeline for identifying high-fitness protein variants with multiple mutations. It accurately selects the best-performing variants using a zero-shot predictor and iterative sampling.

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Last Updated: Jul 2, 2025

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

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Protein Engineering by Yeast Surface Display

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Area of Science:

Protein engineering
Computational biology
Biotechnology

Background:

Assessing protein variant fitness with combinatorial mutations is crucial for protein engineering.
Developing efficient methods to identify high-performing variants is an ongoing challenge.

Purpose of the Study:

To present TopVIP, a novel pipeline for identifying top-performing protein variants.
To enable accurate selection of the greatest number of high-fitness variants.

Main Methods:

Utilizing a zero-shot predictor for initial variant assessment.
Employing low-N iterative sampling for efficient exploration of variant space.

Main Results:

TopVIP enables accurate identification of protein variants with high fitness.
The pipeline effectively selects a large number of best-performing variants.

Conclusions:

TopVIP provides a powerful approach for protein variant selection in protein engineering.
This method enhances the efficiency and accuracy of identifying superior protein designs.