Lnc-PLCB1 is stabilized by METTL14 induced m6A modification and inhibits Helicobacter pylori mediated gastric cancer by destabilizing DDX21
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View abstract on PubMed
Summary
This summary is machine-generated.Helicobacter pylori infection lowers lnc-PLCB1 levels in gastric cancer (GC) via m6A modification. This lncRNA suppresses tumor growth, offering a new therapeutic target for H. pylori-associated GC.
Area Of Science
- Oncology
- Molecular Biology
- Gastroenterology
Background
- Helicobacter pylori (H. pylori) infection is a major risk factor for gastric cancer (GC).
- Long noncoding RNAs (lncRNAs) and N6-methyladenosine (m6A) modification are implicated in GC development.
- The interplay between H. pylori, lncRNAs, and m6A in GC pathogenesis remains largely unexplored.
Purpose Of The Study
- To investigate the role of lncRNA m6A modification in H. pylori-mediated gastric cancer development.
- To elucidate the regulatory mechanism of lnc-PLCB1 in GC progression.
- To identify potential therapeutic targets for H. pylori-associated GC.
Main Methods
- Analysis of lnc-PLCB1 expression in GC tissues and cell lines.
- Investigation of the regulatory effects of H. pylori infection on lnc-PLCB1 expression.
- Assessment of the impact of lnc-PLCB1 overexpression or downregulation on GC cell proliferation and migration.
- Exploration of the molecular interactions involving METTL14, IRF2, lnc-PLCB1, DDX21, CCND1, and Slug.
Main Results
- H. pylori infection downregulates lnc-PLCB1 expression through METTL14-mediated m6A modification and IRF2-mediated transcriptional regulation.
- Overexpression of lnc-PLCB1 inhibits GC cell proliferation and migration, while its downregulation promotes these processes.
- lnc-PLCB1 expression is significantly lower in GC tissues compared to normal tissues.
- lnc-PLCB1 reduces the protein stability of DDX21, subsequently downregulating CCND1 and Slug expression, thereby exerting tumor-suppressive effects.
Conclusions
- The METTL14/lnc-PLCB1/DDX21 axis is crucial in H. pylori-mediated gastric cancer.
- lnc-PLCB1 functions as a tumor suppressor in GC development.
- lnc-PLCB1 represents a promising novel therapeutic target for treating H. pylori-associated gastric cancer.
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