Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

PP2A catalytic subunit alpha is critically required for CD8<sup>+</sup> T-cell homeostasis and antibacterial responses.

European journal of immunology·2024

Same author

Diversity of post-translational modifications and cell signaling revealed by single cell and single organelle mass spectrometry.

Communications biology·2024

Same author

Culture-Free Whole Genome Sequencing of <i>Mycobacterium tuberculosis</i> Using Ligand-Mediated Bead Enrichment Method.

Open forum infectious diseases·2024

Same author

Multicolumn Nanoflow Liquid Chromatography with Accelerated Offline Gradient Generation for Robust and Sensitive Single-Cell Proteome Profiling.

Analytical chemistry·2024

Same author

Metabolome-wide association identifies altered metabolites and metabolic pathways in the serum of patients with cholangiocarcinoma.

JHEP reports : innovation in hepatology·2024

Same author

Dysregulated Cerebrospinal Fluid Proteome of Spinocerebellar Ataxia Type 2 and its Clinical Implications.

Movement disorders : official journal of the Movement Disorder Society·2024

Search research articles

Related Experiment Video

Updated: Jul 2, 2025

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Recent progress in mass spectrometry-based urinary proteomics.

Neha Joshi^1,2,3, Kishore Garapati^1,2,3, Vivek Ghose^1,2

¹Manipal Academy of Higher Education (MAHE), Manipal, 576104, India.

Clinical Proteomics

|February 23, 2024

Summary

This summary is machine-generated.

Urine proteomics offers a non-invasive method for disease biomarker discovery, expanding the catalog of detectable urinary proteins. This approach aids in characterizing diseases and identifying predictive, diagnostic, and prognostic markers.

More Related Videos

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Related Experiment Videos

Last Updated: Jul 2, 2025

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Area of Science:

Biomedical research
Proteomics
Clinical diagnostics

Background:

Serum and plasma require invasive collection, limiting their research utility.
Urine offers a non-invasive alternative for disease characterization and biomarker discovery.
Urinary proteins originate from multiple organs, enabling systemic disease assessment.

Purpose of the Study:

To provide an update on urinary proteomics advancements over the last decade.
To highlight the expanded catalog of urinary proteins identified in various clinical conditions.
To emphasize the growing applications of urine analysis in disease characterization and biomarker discovery.

Main Methods:

Mass spectrometry-based protein profiling of urine.
Proteomic analysis of urine samples.
Review of studies on urinary proteomics from the past decade.

Main Results:

Discovery of several disease-associated biomarkers through urine proteomic analysis.
Expansion of the catalog of proteins detected in urine across diverse clinical conditions.
Demonstration of urine analysis applications beyond kidney and urinary bladder disorders.

Conclusions:

Urinary proteomics is a valuable, non-invasive tool for biomarker discovery and disease characterization.
The scope of urinary proteome analysis has significantly broadened over the past decade.
Continued investigation of the urinary proteome is driven by its potential for predictive, diagnostic, and prognostic marker identification.