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Updated: Jul 17, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Isolation and identification of Methylobacterium komagatae and its application in textile industries
Hridoyjit Phukon1,2, Kumar Harshvardhan2, Neelav Sarma1,2
1Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India.
Abstract:
A light pink-coloured, rod-shaped, gram-negative bacterium isolated from an unproductive crude oil production area was considered as a sample for this study. The 16S rRNA gene sequence identified the isolate as Methylobacterium komagatae. Comparing the standard colour measurement values set by the International Commission on Illumination (CIE) method confirms the colourant produced by the biomass of this microorganism as a 'light pink' colouration. The energy-dispersive X-ray spectroscopy and High-Resolution Mass Spectroscopy process help in the structural elucidation of the sample. It indicates the presence of magnesium (Mg) as a central metal atom in the bacterial colourant, i.e. 'bacteriochlorophyll' (BChl) (MgC55H74N4O). The recovered bacterial colourant was applied to cotton fabric and cotton yarns to dye and examine their fastness quality. The result shows the cotton fabrics retained colourant in normal washing while it got reduced after detergent-based washing. Therefore, its fastness quality must be improved to equalise with current colourants.
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