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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
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Capillary Electrophoresis: Applications01:30

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Desalting Plasma Protein Solutions by Membrane Capacitive Deionization.

Bharat Shrimant1, Tanmay Kulkarni1, Mahmudul Hasan1

  • 1Department of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania 16802, United States.

ACS Applied Materials & Interfaces
|February 23, 2024
PubMed
Summary

Membrane capacitive deionization (MCDI) effectively removes salt ions from plasma protein solutions, offering a promising alternative to traditional methods in biopharmaceutical manufacturing with minimal protein loss.

Keywords:
albuminelectrochemical separationsmembrane capacitive deionizationplasma proteinspoly(phenylene alkylene) ion exchange membranes

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Separation Science
  • Chemical Engineering

Background:

  • Plasma protein therapies are vital for treating numerous diseases.
  • Conventional salt ion removal methods like diafiltration and ion-exchange chromatography face challenges with protein fouling.
  • Efficient ion removal is critical for the purity and efficacy of plasma protein products.

Purpose of the Study:

  • To investigate membrane capacitive deionization (MCDI) as a novel method for salt ion removal from plasma protein solutions.
  • To evaluate the efficacy of MCDI in reducing ion concentration with minimal protein loss.
  • To explore the use of advanced ion exchange membranes (IEMs) for improved MCDI performance.

Main Methods:

  • Applied membrane capacitive deionization (MCDI) to human serum albumin solutions.
  • Utilized highly conductive poly(phenylene alkylene)-based ion exchange membranes (IEMs).
  • Incorporated ionomer-coated nylon meshes in the spacer channel to reduce electrical resistance.

Main Results:

  • Achieved a 28% reduction in salt ions (sodium, chloride, phosphate) from plasma protein solutions.
  • Demonstrated less than 3% protein loss during the MCDI process.
  • Enhanced energy efficiency in MCDI due to improved membrane conductivity and reduced Ohmic resistances.

Conclusions:

  • MCDI is a viable and effective platform for deionizing plasma protein solutions in biopharmaceutical manufacturing.
  • This technique offers a significant advantage over conventional methods by minimizing protein loss.
  • MCDI presents a scalable solution for purifying pharmaceutical formulations without compromising active pharmaceutical ingredients.