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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: May 6, 2026

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Digital PCR for Single-Cell Analysis.

Weibo Fang¹, Xudong Liu¹, Mariam Maiga¹

¹Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, China.

|February 23, 2024

Summary

This summary is machine-generated.

Single-cell analysis reveals cellular heterogeneity using digital polymerase chain reaction (dPCR). Microfluidic dPCR enables precise quantification of single-cell gene expression and proteins, advancing biological and clinical research.

Keywords:

digital PCR microfluidic chip single-cell analysis

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Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

Single-cell analysis is crucial for understanding cellular heterogeneity in biological functions and disease mechanisms.
Digital polymerase chain reaction (dPCR) offers high sensitivity and precision for absolute quantification of nucleic acids and proteins.

Purpose of the Study:

To review the significance and methods of single-cell analysis.
To focus on microfluidic single-cell digital PCR technology and its applications.
To discuss the challenges and opportunities in single-cell dPCR development.

Main Methods:

Single-cell isolation techniques.
Single-cell lysis protocols.
Microfluidic digital PCR for gene expression and protein quantification at the single-cell level.

Main Results:

Digital PCR, particularly microfluidic-based approaches, demonstrates significant advantages for specific gene or protein detection in single cells.
Absolute quantification of single-cell gene expression and proteins is achievable with high sensitivity and precision.

Conclusions:

Microfluidic single-cell dPCR is a powerful tool for advancing biological and clinical research at a single-cell resolution.
Further development in single-cell dPCR holds promise for deeper insights into cellular processes and disease mechanisms.