Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymers

Area of Science:

• Biomedical Engineering
• Materials Science
• Analytical Chemistry

Background:

• Sensitive determination of folate receptors (FRs) is crucial for early cancer detection and management.
• Existing folic acid (FA)-based electrochemical biosensors for FR detection often suffer from complexity, poor reproducibility, biocompatibility issues, and high costs.

Purpose of the Study:

• To develop an environmentally friendly, sensitive, and reliable biosensor for the detection of folate receptors (FRs).
• To address the limitations of current FR detection methods by creating a cost-effective and user-friendly device.

Main Methods:

• Fabrication of an electrochemical impedimetric biosensor using electrospun nanofibers (NFs) composed of bio-copolymers (sodium alginate and polyethylene oxide).
• Incorporation of folic acid (FA) as the biorecognition element onto the NF surface.
• Characterization of NF nanocomposites using SEM, FTIR, zeta potential (ZP), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).

Main Results:

• The developed NF biosensor demonstrated sensitive FR detection in plasma with a low limit of detection (LOD) of 3 pM.
• The biosensor exhibited high selectivity, reliability, and stability over a two-month storage period.
• The use of 'green credentials' polymers aligns with sustainable practices in medical device development.

Conclusions:

• The novel NF-based electrochemical biosensor offers a promising platform for sensitive and selective FR detection.
• This eco-friendly biosensor provides a viable alternative to existing methods, facilitating early cancer diagnosis.
• The biocompatible and stable nature of the biosensor supports its potential application in clinical settings.