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Related Concept Videos

Raman Spectroscopy: Overview01:20

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Surface-Enhanced Spatially Offset Raman Spectroscopy in Tissue.

Dayle Kotturi1, Sureyya Paterson1, Mike McShane1,2

  • 1Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.

Biosensors
|February 23, 2024
PubMed
Summary

Surface-enhanced spatially offset Raman spectroscopy (SESORS) can detect metabolites through tissue barriers. Signal intensity is highest with zero offset, decreasing sharply beyond 2 mm, guiding implantable sensor development.

Keywords:
Monte Carlo modelingSESORSbiocompatiblehydrogelimplantablelight-tissue interactionsurface-enhanced spatially offset Raman spectroscopytissue optics

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Area of Science:

  • Biomedical Optics
  • Spectroscopy
  • Personalized Medicine

Background:

  • Personalized medicine aims for real-time metabolite monitoring to adjust drug dosages.
  • Surface-enhanced spatially offset Raman spectroscopy (SESORS) offers potential for non-invasive, frequent metabolite monitoring under barriers.

Purpose of the Study:

  • To investigate the spatial variation of Surface-Enhanced Raman Spectroscopy (SERS) signal intensity through biological tissue.
  • To develop and validate a computational model for light-tissue interactions in SESORS.

Main Methods:

  • Experimental setup with SERS-active hydrogel implants under varying tissue thicknesses.
  • Monte Carlo simulation incorporating modified optical properties post-inelastic scattering.
  • Collection of emission spectra at the air-tissue boundary across different spatial offsets.

Main Results:

  • Raman signals were detectable through all tested tissue thicknesses.
  • Maximum signal intensity observed at 0 mm offset (co-localized excitation and detection).
  • Signal intensity decreased significantly beyond 2 mm offset, with model and experimental data showing agreement.

Conclusions:

  • SESORS signal intensity is highly dependent on the spatial offset between excitation and detection.
  • The developed model accurately predicts SESORS signal behavior, aiding in optimal sensor design.
  • Findings support the feasibility of implantable SESORS for metabolite monitoring, informing future device development.