Agreement between Ventilatory Thresholds and Bilaterally Measured Vastus Lateralis Muscle Oxygen Saturation...

This study focused on comparing metabolic thresholds derived from local muscle oxygen saturation (SmO₂) signals, obtained using near-infrared spectroscopy (NIRS), with global pulmonary ventilation rates measured at the mouth. It was conducted among various Age Groups within a well-trained cyclist population. Additionally, the study examined how cycling performance characteristics impact the discrepancies between ventilatory thresholds (VTs) and SmO₂ breakpoints (BPs).

Junior (n = 18) and Senior (n = 15) cyclists underwent incremental cycling tests to assess their aerobic performance and to determine aerobic (AeT) and anaerobic (AnT) threshold characteristics through pulmonary gas exchange and changes in linearity of the vastus lateralis (VL) muscle SmO₂ signals. We compared the relative power (Pkg) at ventilatory thresholds (VTs) and breakpoints (BPs) for the nondominant (ND), dominant (DO), and bilaterally averaged (Avr) SmO₂ during the agreement analysis. Additionally, a 30 s sprint test was performed to estimate anaerobic performance capabilities and to assess the cyclists' phenotype, defined as the ratio of P@VT2 to the highest 5 s sprint power.

The P_kg@BP for Avr SmO₂ had higher agreement with VT values than ND and DO. Avr SmO₂ P_kg@BP1 was lower (p < 0.05) than P_kg@VT1 (mean bias: 0.12 ± 0.29 W/kg; Limits of Agreement (LOA): -0.45 to 0.68 W/kg; R² = 0.72) and mainly among Seniors (0.21 ± 0.22 W/kg; LOA: -0.22 to 0.63 W/kg); there was no difference (p > 0.05) between Avr P_kg@BP2 and P_kg@VT2 (0.03 ± 0.22 W/kg; LOA: -0.40 to 0.45 W/kg; R² = 0.86). The bias between two methods correlated significantly with the phenotype (r = -0.385 and r = -0.515 for AeT and AnT, respectively).

Two breakpoints can be defined in the NIRS-captured SmO₂ signal of VL, but the agreement between the two methods at the individual level was too low for interchangeable usage of those methods in the practical training process. Older cyclists generally exhibited earlier thresholds in muscle oxygenation signals compared to systemic responses, unlike younger cyclists who showed greater variability and no significant differences in this regard in bias values between the two threshold evaluation methods with no significant difference between methods. More sprinter-type cyclists tended to have systemic VT thresholds earlier than local NIRS-derived thresholds than athletes with relatively higher aerobic abilities.