Exposure to the plasticizer dibutyl phthalate causes oxidative stress and neurotoxicity in brain tissue

The phthalate ester, dibutyl phthalate (DBP), is one of the endocrine-disrupting chemicals detected in various aquatic environments. Previous research has found multiple toxic effects of DBP in aquatic organisms; however, the neurotoxic effects of the compound are surprisingly scanty. The purpose of this study was aimed to evaluate the role of oxidative stress in the induction of neurotoxicity in the brain tissue of the fish Pseudetroplus maculatus. The fish were exposed to the sublethal concentration of DBP (200 µg L^-1) for 1, 4, 7, and 15 days along with control and vehicle control groups. The induction of oxidative stress in the brain subcellular fractions was proved by alterations in the activities of superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase along with the reduction in the total antioxidant capacity. Meanwhile, the levels of hydrogen peroxide and lipid peroxidation were increased. Neurotransmitters such as acetylcholine, dopamine, adrenaline, noradrenaline, and serotonin were altered in all subcellular fractions suggesting the disruption of the neurotransmitter system in the fish brain. These results indicate that DBP induces oxidative stress, which correlates with neurotoxicity in Pseudetroplus maculatus brain tissue.